CREW continues to make progress in improving the success of artificial insemination (AI) for propagating endangered
cats. In recent research, we incorporated treatment with oral progesterone (Regumate) into our AI protocol for domestic cats to down-regulate ovarian function prior to ovarian stimulation. This approach allows us to control ovarian activity more precisely and conduct AI procedures on a fixed time schedule.
Our first attempt using this method in exotic felids involved our fishing cat named Ratana,who was incapable of breeding naturally after losing a front leg due to injury. Ratana was fed a small amount of oral progesterone daily for one month to suppress her ovarian activity and then treated with gonadotropins to induce follicular growth and ovulation. Laparoscopic AI of both oviducts with freshly collected sperm from our resident male, named Gorton, resulted in conception and the birth of a male fishing cat kitten after a 69 day gestation.
This kitten was the first non-domestic cat born following the use of oral progesterone for fixed time AI, and represents the fifth cat species (fishing cat, ocelot, Pallas’ cat, tiger, domestic cat) that we have produced with oviductal AI. This new approach could greatly advance our capacity to use AI for the genetic management of endangered felid species.