Category — CREW
Scientists at CREW are studying the reproduction of red pandas and have diagnosed pregnancies via trans-abdominal ultrasound. However, performing diagnostic ultrasound imaging requires animal training, a costly ultrasound machine (and a trained ultrasonographer to use it), and is not easily performed on less agreeable individuals. The development of a pregnancy test based on fecal analysis would allow non-invasive pregnancy detection in any female and also could be applied to wild individuals.
In addition to performing regular ultrasounds on the Zoo’s female red pandas, Bailey and Idgie (who has since transferred to another zoo), CREW scientists are measuring fecal hormone metabolites, such as progesterone (P4), to assess their usefulness as indicators of pregnancy.
Bailey had cubs in 2012 and 2013, and both pregnancies were diagnosed via ultrasound. As expected, fecal hormone metabolite analysis showed that her P4 concentrations increased after breeding and remained elevated until she gave birth. The other female, Idgie, was observed breeding, but no pregnancies were detected. Fecal P4 analysis revealed that her P4 was actually higher than Bailey’s in both years, even though she was not pregnant.
These data support the theory of pseudo-pregnancy in red pandas, which has been suggested for years, but not yet proven. Although P4 is generally considered to be the “pregnancy hormone” and can be used to infer pregnancy status in many species, these results indicate that P4 levels alone cannot be used to diagnose pregnancy in red pandas.
December 17, 2014 No Comments
According to Elvis, it’s going to be another disappointing year for polar bear births. There is no pregnancy test for polar bears, but Elvis, a 3-year old beagle who lives at IronHeart High Performance Working Dogs, is being evaluated on his ability to diagnose pregnancy by smelling fecal samples (to read more about Elvis and his training, click here). Polar bears experience low reproductive rates world-wide but are exceptionally challenging to study because traditional methods of pregnancy detection, such as progesterone analysis, don’t distinguish pregnancy from pseudo-pregnancy this species. The 17 potential polar bear moms involved in this year’s Elvis study reside in zoos as close as Columbus and as far as Copenhagen. Last year, Elvis was 93% accurate in his pregnancy predictions. If his diagnostic accuracy is similar to last year, we’ll be lucky if just one or two bears have cubs.
But while Elvis sniffs poop, scientists at the Center for Conservation and Research of Endangered Wildlife (CREW) are using cutting-edge research to figure out which components of a fecal sample Elvis might be recognizing in an effort to develop a laboratory-based pregnancy test. A pregnancy test would allow them to determine where the reproductive process is failing so that potential causes can be addressed. A polar bear pregnancy test wouldn’t just be useful for bears in zoos- it could also help their wild cousins. Since the test would rely on a fecal sample, it could be used to non-invasively monitor wild populations of polar bears, whose numbers are predicted to decline.
Unfortunately, the Elvis test showed that “Berit”, the Cincinnati Zoo’s female is not pregnant again this year and so far, there’s been no other word of cub arrivals. Polar bears can give birth anytime from October to January, so Elvis will need to wait a few more weeks to find out how he performed.
To make a donation to CREW’s polar bear research, please visit the Polar Bear Challenge webpage. Donations made by Dec 31st will be matched dollar for dollar by the Young Family Foundation.
December 10, 2014 2 Comments
Welcoming Two New Post-Doctoral Fellows
Two new post-doctoral fellows, Dr. Lindsey Vansandt and Dr. Anne-Catherine Vanhove, were welcomed to CREW in the fall of 2014.
With funding support from the Joanie Bernard Foundation, Dr. Vansandt will be working with Dr. Bill Swanson, CREW’s Director of Animal Research. Dr. Vansandt obtained her Doctor of Veterinary Medicine degree from the University of Missouri and her Doctor of Philosophy degree from the University of Maryland (in collaboration with the Smithsonian Conservation Biology Institute). Her Ph.D. studies focused on characterization and propagation of spermatogonial stem cells in domestic cats as a model for conserving endangered cat species. Dr. Vansandt also has experience working in veterinary emergency services. At CREW, she will be conducting studies to improve the health and welfare of feral and shelter cats as well as helping to apply oviductal AI for propagation of endangered felids.
With funding from the Institute of Museum and Library Services (IMLS), Dr. Vanhove will be evaluating survival of plant samples in CREW’s Frozen Garden under the supervision of Dr. Valerie Pence, Director of Plant Research. Dr. Vanhove will complete the second phase of the IMLS project, focusing primarily on the survival of shoot tips and gametophytes after long-term storage in liquid nitrogen. She recently received her Ph.D. from the Division of Crop Biotechnics, Katholieke Universiteit Leuven, in Leuven, Belgium. Her thesis work with meristem culture, stress physiology, and cryopreservation makes her well suited for the IMLS project.
The University of Cincinnati/Cincinnati Zoo Connection
CREW has had a long-standing collaborative relationship with the University of Cincinnati’s (UC) Department of Biological Sciences, but today it is strengthened by two promising young scientists who split their time between CREW and UC. Corrina DeLorenzo and Megan Philpott are both enrolled in UC’s Ph.D. program under Drs. Ken Petren and Theresa Culley, respectively, but they are conducting much of their dissertation research at CREW.
Corrina earned her bachelor’s degree at Miami University, with a double major in Zoology and Environmental Science. As an undergraduate, she became involved in research evaluating the population genetics of the Italian wall lizard or “Lazarus lizard” in the Cincinnati area. After graduating, Corrina was accepted to CREW’s summer internship program, working with Dr. Erin Curry on the Polar Bear Signature Project. She was recruited into UC’s graduate program in January 2014. Since starting her Ph.D. research, Corrina has identified multiple antibodies that detect specific proteins in polar bear feces in an effort to develop a polar bear pregnancy test.
Megan received her bachelor’s degree from UC in Biology and was also an intern at the Cincinnati Museum Center, managing the Museum’s Philippine Bird Genetics project. Her Ph.D. research is part of the Plant Lab’s IMLS funded project to evaluate samples that have been stored for years in CREW’s CryoBioBank for genetic changes that might have occurred over time. In April, Megan was awarded the Botanical Society of America’s Public Policy award to attend Congressional Visits Day on Capitol Hill. There, she learned about communicating science to policy makers and met with the offices of Ohio Senators and Representatives to request their support for increased federal funding of scientific research, using CREW’s research as an example of the importance of federal funding and support. (Students supported by the UC Department of Biological Sciences, Institute of Museum and Library Services and CREW Eisenberg Fellowship.)
P&G Wildlife Conservation Scholars
In 2011, CREW established a partnership with the Ohio State University’s College of Veterinary Medicine to train veterinary students in conservation sciences with funding support from Procter & Gamble Pet Care. This past summer, two OSU veterinary students, Kelly Vollman and JaCi Johnson, were selected as P&G Wildlife Conservation Scholars.
Kelly worked with Dr. Monica Stoops analyzing urinary testosterone and glucocorticoid concentrations to determine if the pattern of excretion could be used to predict gender, parturition date and assess fetal viability during Indian rhino gestation. Kelly analyzed urine samples collected throughout seven Indian rhino pregnancies that resulted in three male and four female calves. Six of the pregnancies ended with the birth of live calves, whereas one pregnancy ended in a stillbirth, a relatively common occurrence in this rhino species. By learning more about the endocrinology of pregnancy, results from Kelly’s study will help establish physiological markers to improve pregnancy outcome in this species.
JaCi worked with Dr. Bill Swanson to investigate cat sperm vitrification as an alternative to standard slow freezing methods. Vitrification involves ultra-rapid cooling to avoid ice crystal formation and form a “glass” instead. For this study, JaCi collected semen from domestic cats (and one ocelot) and compared vitrification in a sucrose solution, with direct pelleting in liquid nitrogen, to slow freezing with glycerol in straws over liquid nitrogen vapor. Post-thaw sperm motility and acrosome status were similar between methods and 25% of domestic cat oocytes were fertilized following insemination with vitrified
sperm. This simplified approach to cat semen preservation may be particularly useful for field biologists working with felids in the wild.
November 21, 2014 No Comments