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Category — plant research

One Year After First Outplanting of CREW Propagated Ferns in Florida

Florida has more fern species than any other state in the mainland United States, and CREW is working with partners at Fairchild Tropical Botanic Garden in Miami, Florida, to help propagate several of the most endangered from rapid urbanization and habitat loss.

The gridscale maiden fern, Thelypteris patens, is a large, beautiful fern that can reach over five feet in height. The patens variety is known only from the pine rockland habitat in Miami-Dade County and is listed as endangered in Florida. The population in one particular preserve declined to a single plant, which died in 2013.

Thelypteris patens (Photo: Daderot)

Thelypteris patens (Photo: Daderot)

However, before it died, researchers from Fairchild Tropical Botanic Garden collected spores from that plant and sent them to CREW’s Plant Lab here at the Cincinnati Zoo & Botanical Garden. CREW plant scientists germinated the spores in test tubes (in vitro) to produce tiny gametophytes, which represent the first stage in the fern life cycle.

Growing T. patens in test tubes at CREW

Growing T. patens in test tubes at CREW

These were then nurtured further in culture to produce sporophytes, which represent the second stage of fern growth and are the plants we normally think of as ferns. The sporophytes were acclimatized to soil at CREW, and then over 200 of these plants were sent to collaborators at Fairchild Tropical Botanic Garden where they were grown further in their greenhouses.

T. patens

T. patens

Finally, in May and June of last year, over 150 of the ferns propagated at CREW were outplanted back into the preserve where the species had been extirpated.

Outplanting T. patens in Florida

Outplanting T. patens in Florida

The plants are being monitored by Fairchild Tropical Botanic Garden. As of February 2015, the plants have had an 89% survival rate.

June 23, 2015   No Comments

Saving Animals in the Wild: The Zoo’s Top Field Conservation Efforts of 2014

Happy New Year! As we look back on 2014, let’s reflect on some of the Zoo’s significant contributions to wildlife conservation in the field this past year:

Nasha and cubs snoozing on the  savannah

Nasha and cubs snoozing on the savannah

Helping Lions Thrive in Kenya’s South Rift Valley

Since 2011, the Zoo has partnered with the African Conservation Centre and the South Rift Association of Land Owners in Kenya on the Rebuilding the Pride program. This community-based conservation program combines Maasai tradition and modern technology to restore a healthy lion population while reducing the loss of livestock to lions in Kenya’s South Rift Valley. Once down to a low of about 10 known lions in the area, the population has grown to more than 65 lions in 2014. This past April, a lioness named Nasha gave birth to another litter, this one containing three cubs. That the population is growing in the South Rift Valley at a time when lion populations are severely declining across the continent overall is significant and a testament to program’s community-based approach.

Sumatran rhinos at the Sumatran Rhino Sanctuary in Indonesia

Sumatran rhinos at the Sumatran Rhino Sanctuary in Indonesia

A Giant Step Forward for Sumatran Rhinos in the Wild

The Zoo has been committed to saving the Sumatran rhino for 25 years. Despite the devastating blow of the loss of our female rhino, Suci, back in March, the Zoo continues to work to conserve and protect the species. In 2014, a Debt-for-Nature deal was struck between the United States and Indonesia. In return for lowering the debt Indonesia owes to the United States, it will commit nearly $12 million towards the conservation and protection of critically endangered species, including the Sumatran rhino, and their habitats over the next seven years. The debt swap was made possible by a contribution of about $11.2 million from the U.S. government under the Tropical Forest Conservation Act (first introduced by Ohio Senator Rob Portman in 1998) and $560,000 from other organizations funneled through Conservation International. The Zoo was proud to help secure this funding by pledging a major gift.

Pollinating American chestnut trees with cryopreserved pollen

Pollinating American chestnut trees with cryopreserved pollen

Saving American Chestnut Trees with Cryopreserved Pollen

The magnificent American chestnut tree once ranged over the entire Eastern United States, but was almost entirely obliterated by blight by the mid-twentieth century. Over the years, breeders have been working to develop a resistant tree, and one of their key tools is pollen. American chestnut pollen rapidly declines in viability so maintaining important lines of pollen from year to year is difficult. In 1993, pollen was cryopreserved (frozen) in liquid nitrogen at the Zoo’s Center for Conservation and Research of Endangered Wildlife (CREW). Last spring, some of that pollen was removed and used to successfully pollinate trees at the American Chestnut Foundation’s farm in Virginia. Paternity testing will be done at CREW this winter, but the indications are very good that cryopreservation can indeed maintain pollen viability for at least 20 years—a fact that should provide a new tool to those working to save this majestic American tree.

Kea Conservation Trust staff conducting kea research (Photo: Nigel Adams)

Kea Conservation Trust staff conducting kea research (Photo: Nigel Adams)

Committing to Kea Conservation in New Zealand

Over the past few years, the Zoo has supported the Kea Conservation Trust’s (KCT) efforts to protect and study New Zealand’s mountain parrot, the kea, in the wild. In 2014, the Zoo stepped up its efforts with a commitment to support the Kea-Community Conflict Response Plan, a multi-year proactive community-focused conflict response and resolution program. Funds from the Zoo support a key personnel position, the Community Volunteers Coordinator, who can respond to conflict situations that arise. Funds will also enable KCT staff to enhance their conflict resolution skills by participating in a Human-Wildlife Conflict Collaboration Workshop. Additionally, Zoo aviculturists will join the KCT team for kea nest monitoring and field work over the next couple of years.

Dr. Alan Rabinowitz, CEO of Panthera, a global wild cat conservation organization, installs a camera trap in Bhutan. (Photo: Steve Winter)

Dr. Alan Rabinowitz, CEO of Panthera, a global wild cat conservation organization, installs a camera trap in Bhutan. (Photo: Steve Winter)

Pledging Support for Panthera’s Tigers Forever Initiative

The Zoo is committed to ensuring the survival of endangered tigers of which there are fewer than 3,200 remaining in the wild. In 2014, we have pledged multi-year support of the tiger conservation efforts of Panthera, the leading international wild cat conservation organization with a mission to ensure the future of wild cats through scientific leadership and global conservation action. To ensure the tiger’s survival, Panthera works across Asia with numerous partners to end the poaching of tigers for the illegal wildlife trade, prevent tiger deaths due to conflict with humans and livestock, and protect tiger prey species and habitat. Through their program, Tigers Forever, Panthera works to protect and secure key tiger populations and ensure connectivity between sites so that tigers can live long into the future.

Wetlands in restoration at EcOhio Farm

Wetlands in restoration at EcOhio Farm

Restoring Native Wetlands and Wildlife in Mason, Ohio

In 1995, a 529-acre farm in Mason, Ohio, now called the EcOhio Farm, was willed to the Zoo with the guideline that it could never be developed unless it is to further the mission of the Zoo. Over the past few years, the Zoo has worked to restore 30 of the farm’s acres to its original state of a wet sedge meadow, providing refuge for a diversity of native wildlife. Since restoration began in 2012, drainage tiles have been removed and more than 200 native plant species and thousands of trees have been planted on the site. The wetland is returning to its natural state very quickly. Already, it has attracted more than 135 native bird species, including bald eagles, bobolinks and killdeer, which would never have been there when it was a cornfield. Though not currently open to the public, walking trails and a small education center may be implemented in the future to provide opportunities to explore the wetland.

Cincinnati Zoo gorillas, Asha and  her infant, Mondika, named for the field conservation project (Photo: Michelle Curley)

Cincinnati Zoo gorillas, Asha and her infant, Mondika, named for the field conservation project (Photo: Michelle Curley)

Strengthening our Support for Gorillas in the Republic of Congo

Over the past 20 years, the Zoo has partnered with the Nouabale Ndoki Project (NNP) in the Republic of Congo, which includes the Mbeli Bai Study, the longest running study of the critically endangered western lowland gorilla. The Zoo also supports work in an area called Mondika where gorillas are habituated for up close research and ecotourism. Habituation is a process through which the wild gorillas become accustomed to and tolerate the presence of people that enables researchers and tourists to observe them up close. The Zoo recently helped facilitate the habituation of a second group of gorillas, and entered into an agreement in 2014 to support the habituation of a third group over the next three years. Special thanks goes to Gorilla Glue, our official gorilla conservation sponsor!

All of these projects and more couldn’t happen without your support of the Zoo. Here’s to you and the Zoo making more great strides for wildlife conservation in 2015!

January 7, 2015   No Comments

Meet Some New Faces at CREW

Welcoming Two New Post-Doctoral Fellows

Two new post-doctoral fellows, Dr. Lindsey Vansandt and Dr. Anne-Catherine Vanhove, were welcomed to CREW in the fall of 2014.

With funding support from the Joanie Bernard Foundation, Dr. Vansandt will be working with Dr. Bill Swanson, CREW’s Director of Animal Research. Dr. Vansandt obtained her Doctor of Veterinary Medicine degree from the University of Missouri and her Doctor of Philosophy degree from the University of Maryland (in collaboration with the Smithsonian Conservation Biology Institute). Her Ph.D. studies focused on characterization and propagation of spermatogonial stem cells in domestic cats as a model for conserving endangered cat species. Dr. Vansandt also has experience working in veterinary emergency services. At CREW, she will be conducting studies to improve the health and welfare of feral and shelter cats as well as helping to apply oviductal AI for propagation of endangered felids.

Lindsey Vansandt, DVM

Lindsey Vansandt, DVM

With funding from the Institute of Museum and Library Services (IMLS), Dr. Vanhove will be evaluating survival of plant samples in CREW’s Frozen Garden under the supervision of Dr. Valerie Pence, Director of Plant Research. Dr. Vanhove will complete the second phase of the IMLS project, focusing primarily on the survival of shoot tips and gametophytes after long-term storage in liquid nitrogen. She recently received her Ph.D. from the Division of Crop Biotechnics, Katholieke Universiteit Leuven, in Leuven, Belgium. Her thesis work with meristem culture, stress physiology, and cryopreservation makes her well suited for the IMLS project.

Anne-Catherine Vanhove

Anne-Catherine Vanhove

The University of Cincinnati/Cincinnati Zoo Connection

CREW has had a long-standing collaborative relationship with the University of Cincinnati’s (UC) Department of Biological Sciences, but today it is strengthened by two promising young scientists who split their time between CREW and UC. Corrina DeLorenzo and Megan Philpott are both enrolled in UC’s Ph.D. program under Drs. Ken Petren and Theresa Culley, respectively, but they are conducting much of their dissertation research at CREW.

Corrina earned her bachelor’s degree at Miami University, with a double major in Zoology and Environmental Science. As an undergraduate, she became involved in research evaluating the population genetics of the Italian wall lizard or “Lazarus lizard” in the Cincinnati area. After graduating, Corrina was accepted to CREW’s summer internship program, working with Dr. Erin Curry on the Polar Bear Signature Project. She was recruited into UC’s graduate program in January 2014. Since starting her Ph.D. research, Corrina has identified multiple antibodies that detect specific proteins in polar bear feces in an effort to develop a polar bear pregnancy test.

Corrina DeLorenzo

Corrina DeLorenzo

Megan received her bachelor’s degree from UC in Biology and was also an intern at the Cincinnati Museum Center, managing the Museum’s Philippine Bird Genetics project. Her Ph.D. research is part of the Plant Lab’s IMLS funded project to evaluate samples that have been stored for years in CREW’s CryoBioBank for genetic changes that might have occurred over time. In April, Megan was awarded the Botanical Society of America’s Public Policy award to attend Congressional Visits Day on Capitol Hill. There, she learned about communicating science to policy makers and met with the offices of Ohio Senators and Representatives to request their support for increased federal funding of scientific research, using CREW’s research as an example of the importance of federal funding and support. (Students supported by the UC Department of Biological Sciences, Institute of Museum and Library Services and CREW Eisenberg Fellowship.)

Megan Philpott

Megan Philpott

P&G Wildlife Conservation Scholars

In 2011, CREW established a partnership with the Ohio State University’s College of Veterinary Medicine to train veterinary students in conservation sciences with funding support from Procter & Gamble Pet Care. This past summer, two OSU veterinary students, Kelly Vollman and JaCi Johnson, were selected as P&G Wildlife Conservation Scholars.

Kelly worked with Dr. Monica Stoops analyzing urinary testosterone and glucocorticoid concentrations to determine if the pattern of excretion could be used to predict gender, parturition date and assess fetal viability during Indian rhino gestation. Kelly analyzed urine samples collected throughout seven Indian rhino pregnancies that resulted in three male and four female calves. Six of the pregnancies ended with the birth of live calves, whereas one pregnancy ended in a stillbirth, a relatively common occurrence in this rhino species. By learning more about the endocrinology of pregnancy, results from Kelly’s study will help establish physiological markers to improve pregnancy outcome in this species.

Kelly Vollman

Kelly Vollman

JaCi worked with Dr. Bill Swanson to investigate cat sperm vitrification as an alternative to standard slow freezing methods. Vitrification involves ultra-rapid cooling to avoid ice crystal formation and form a “glass” instead. For this study, JaCi collected semen from domestic cats (and one ocelot) and compared vitrification in a sucrose solution, with direct pelleting in liquid nitrogen, to slow freezing with glycerol in straws over liquid nitrogen vapor. Post-thaw sperm motility and acrosome status were similar between methods and 25% of domestic cat oocytes were fertilized following insemination with vitrified
sperm. This simplified approach to cat semen preservation may be particularly useful for field biologists working with felids in the wild.

JaCi Johnson

JaCi Johnson

November 21, 2014   No Comments