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Category — General Zoo

Successful Fixed Time Artificial Insemination in the Fishing Cat

CREW continues to make progress in improving the success of artificial insemination (AI) for propagating endangered
cats. In recent research, we incorporated treatment with oral progesterone (Regumate) into our AI protocol for domestic cats to down-regulate ovarian function prior to ovarian stimulation. This approach allows us to control ovarian activity more precisely and conduct AI procedures on a fixed time schedule.

Dr. William Swanson performs an AI procedure.

Dr. William Swanson performs an AI procedure.

Our first attempt using this method in exotic felids involved our fishing cat named Ratana,who was incapable of breeding naturally after losing a front leg due to injury. Ratana was fed a small amount of oral progesterone daily for one month to suppress her ovarian activity and then treated with gonadotropins to induce follicular growth and ovulation. Laparoscopic AI of both oviducts with freshly collected sperm from our resident male, named Gorton, resulted in conception and the birth of a male fishing cat kitten after a 69 day gestation.

Ratana and her kitten in her nest box

Ratana and her kitten in her nest box

This kitten was the first non-domestic cat born following the use of oral progesterone for fixed time AI, and represents the fifth cat species (fishing cat, ocelot, Pallas’ cat, tiger, domestic cat) that we have produced with oviductal AI. This new approach could greatly advance our capacity to use AI for the genetic management of endangered felid species.

Fishing cat (Photo: Connie Lemperle)

Fishing cat (Photo: Connie Lemperle)

April 11, 2014   1 Comment

Breeding North American River Otters

north_american_river_otter_ faceCharismatic and curious North American river otters are so much fun to watch, but to get them to breed? That’s a whole lot trickier! North American river otters, as their name would suggest, live as far south as the Florida everglades, throughout most of the lower 48 states all the way north to the sub-arctic regions of US (Alaska) and Canada.  For these otters, when to breed and give birth needs to be timed so the pups are born when it’s not too cold and there are plenty of resources for mom and pup(s) to thrive.  Basically, otters in the south give birth and breed much earlier in the season than otters living further north.

In addition, female otters undergo a process known as delayed implantation. Fertilized otter eggs start developing into embryos, but stop developing for 7-10 months until they are eventually triggered to implant into the uterine wall and develop once again. The female otter gives birth (litter size 1-4 pups) approximately 70 days after implantation starts. Delayed implantation is what, in part, helps river otters time when they give birth.  So how does one know when implantation has occurred and when their otter may give birth? — By monitoring the hormones in their poop!

Each year starting usually November through January/February, US Zoos send otter fecal samples to CREW hoping to figure out if their otter may be pregnant and when she may be due. This season we monitored fecal samples from 11 river otter females and we were able to determine 8 of these females were possibly pregnant and 6 of those have already given birth {for example: pueblo zoo, buffalo Zoo}, making it a banner year for river otter babies!

Pueblo Zoo Otter Pup

Pueblo Zoo Otter Pup

However, the story doesn’t end there. Our research has shown that female otters born further south and moved to another zoo much further north, don’t always respond to the environmental cues encountered at their new latitude and adjust when they should breed and give birth – making it hard to know when to pair otters for breeding – but those fecal hormones have also proven useful in determining when to pair up otters as well {North Carolina}.

Publeo Zoo Pups – On Zooborns

Buffalo Zoo Pups – On Facebook

North Carolina Pups – News Article

March 28, 2014   No Comments

Zoo Volunteer Observers

Our Zoo Volunteer Observers (ZVO) are a great asset to the Zoo &  our Carl H. Lindner Jr. Family Center for Conservation and Research of Endangered Wildlife (CREW) – being our eyes (and ears sometimes) when keepers go home at night.  They have been busy lately monitoring polar bear breeding activity and watching our pregnant giraffe via web cam 24-hrs-day for signs of labor.

nancy_donna.jpg

Volunteers Nancy W. and Donna M. watching the polar bears.

The ZVOs have been monitoring the polar bears daily to look for signs of impending estrus/breeding behavior. Their diligent observations are helping CREW researchers learn more about polar bear reproduction and reproductive behavior.

Thank you ZVOs!

March 21, 2014   2 Comments